Aconitase levels in the leaves of iron-deficient mustard plants (Sinapis alba)
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چکیده
منابع مشابه
Nucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba).
From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (32...
متن کاملDiurnal Rhythmicity in the Pattern of mRNAs in the Leaves of Sinapis alba.
Previous studies have shown that certain specific leaf mRNAs exhibit a diurnal rhythmicity in their quantity in higher plants. To determine whether this situation is restricted to a few mRNAs, or affects a large number, we have used in vitro translation and two-dimensional polyacrylamide gel electrophoresis to analyze the mRNA complement in leaves of Sinapis alba at different times during an 8-...
متن کاملSequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba).
Plant chloroplasts contain transcription factors that functionally resemble bacterial sigma factors. We have cloned the full-length cDNA from mustard (Sinapis alba) for a 53 kDa derived polypeptide that contains similarity to regions 1.2-4.2 of sigma70-type factors. The amino acid sequence at the N-terminus has characteristics of a chloroplast transit peptide. An in vitro synthesized polypeptid...
متن کاملCharacterization of Glutamine Synthetase of Roots, Etiolated Cotyledons and Green Leaves from Sinapis alba (L.)
Glutamine synthetase of roots, etiolated cotyledons and green leaves from mustard plants cannot all clearly be separated by DEAE-Sephacel chromatography. However, the enzyme of the roots, etiolated cotyledons and green leaves, respectively, differed in the kinetic properties deter mined in the crude extract. The root enzyme showed a pH-optimum of about 6.9, a Km value of 3 m M for glutamate an...
متن کاملDevelopment and validation of a real-time PCR method for the detection of white mustard (Sinapis alba) in foods.
This paper presents a real-time PCR method allowing the detection of traces of white mustard ( Sinapis alba ) in complex food matrices. The primers and the probe are targeted at the gene coding for S. alba MADS D. The real-time PCR method was found to be specific for white mustard and did not show any cross-reactivity with 67 biological species, including 12 members of the Brassicaceae family. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1961
ISSN: 0006-2936
DOI: 10.1042/bj0800064